E-mail address: wasghar fau. Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. Conventional semen analysis methods involve complex and manual inspection with a microscope, and these methods are labor intensive and can take several days. Furthermore, as conventional methods have to be conducted in the laboratories, many men are unwilling to be tested as a result of social stigma in certain regions of the world.
If motility is Zerothen pentoxyphillene stimulation test is required Figures References Related Information. The role of strict sperm morphology became evident in the s as illustrated in the following graphic 31 Notice in this example that the man in each pair has a score of three Notice in this Powerpoint presentation physiology labs semen analysis that the man in each pair has a score of three. Then, they must attach the device to the Powerpojnt, transfer the sample to a slide using a pipette, and then insert the slide into the device. From this document you are allowed to analyse and export detailed track data. Vital staining — determines numbers of living and dead sperm. The degree of similarity physioloogy the sperm head to a circle.
Marc anthony bulge. Introduction
Morphometry : Morphometry The image analyzer is used to perform the measurement of architecturalcellular and nuclear features of the cells. On-line Supplement. Chart and Diagram Slides for PowerPoint - Beautifully designed chart and diagram s for PowerPoint with visually stunning graphics and animation effects. The scans for fluorescent signals emmitted and Powerpoint presentation physiology labs semen analysis bioinformatics for to see level of gene expression and genotyping finding differences in genes. Hematopoiesis 5. Anagha VNAyurvedic Physician. Introduction to anemia That's all free as well! Share a link to All Resources. The area of convergence is the point of origin the spot where the blow occurred.
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Thank you! Published by Edith Delilah Shelton Modified over 3 years ago. Concerns about the size of genital are often unwarranted. Other researcher say it does. Because of a genetic signal causes the penis cells to die off during gestation.
A court has allowed the parents of a dead man to chose a woman to be impregnated by his sperm, which is stored in a similar facility. Photo by Moti Milrod. The fluid is examined for the presence of motile sperm and then sent to the andrology laboratory for cryopreservation Microsurgical Epididymal Sperm Aspiration.
Microdissection Testicular Sperm Extraction. Sperm counts peak at two to three days after ejaculation, meaning, after you have sex or masturbate, the swimmers will be most abundant two to three days later. Sperm live for about 48 hours in the female reproductive tract, so the optimum timing is sex every other day. Studies show that conception usually happens up to six days before, and ending on, ovulation.
The big problem is that all tests for ovulation show the event after it happens. So a good plan is start having sex every other day after the beginning of menstruation by 9 days, and keep going every other day for 5 times the possible days of ovulation.
Then be at your ease until the next menstruation. Over 15 million sperm per milliliter is considered normal, according to the WHO in Older definitions state 20 million. A lower sperm count is considered oligozoospermia. Morphology is a predictor of success in fertilizing oocytes during in vitro fertilization.
Also, sperm cells with tail-tip swelling patterns generally have lower frequency of aneuploidy. Volume Web MD advises that semen volumes between 1. Low volume may indicate partial or complete blockage of the seminal vesicles, or that the man was born without seminal vesicle. Absence of fructose may indicate a problem with the seminal vesicles.
A basic ejaculate higher pH value may indicate an infection. Liquefaction The liquefaction is the process when the gel formed by proteins from the seminal vesicles is broken up and the semen becomes more liquid. It normally takes less than 20 minutes for the sample to change from a thick gel into a liquid.
Thus, it is a combination of sperm count and motility. Others The sample may also be tested for white blood cells. A high level of white blood cells in semen is called leucospermia and may indicate an infection. Most systems are based on image analysis, but alternative methods exist such as tracking cell movement on a digitizing tablet. Computer-assisted techniques are most-often used for the assessment of sperm concentration and mobility characteristics, such as velocity and linear velocity.
Nowadays, there are CASA systems, based on image analysis and using new techniques, with near perfect results, and doing full analysis in a few seconds. With some techniques, sperm concentration and motility measurements are at least as reliable as current manual methods.
Raman spectroscopy has made progress in its ability to perform the characterization 7 the perform of DNA damage. Kruger Strict analysis requires that each apparently normal sperm be measured for head size. According to Kruger, the head length for normal spermatozoa may vary between 4. The length to width ratio should be about 1. Any abnormalities in the neck, midpiece or tail also automatically render the sperm abnormal regardless of head quality. The normal midpiece length is 1.
Is it any wonder that most sperm techs hate this tedious test? What is the good news for patients with poor morphology results? Very low numbers of normal forms may suggest that IVF or ICSI may be necessary, but technical treatments are possible to overcome even very poor morphology results. Head: a. Nucleus: contain genetic code b. Postnuclear cap: cover posterior portion of nucleus c. Acrosome: cover anterior portion of nucleus and contain enzymes as hyaluronidase,corona penetrating enzyme and acrosin B.
Neck: sometimes included in "Tail" Proximal centriole: join head and tail, where head and tail separate during fertilization and in heat-damaged semen C.
Tail: a. Mid-piece: Mitochondrial sheath: contain enzymes converting energy substrates as fructose into ATP b. Function: Serve as buffer, optimal osmotic and nutrient medium B. Osmotic pressure: similar to blood and physiological saline 0. Energy substrates: fructose, sorbitol etc Inorganic ions A.
Function: Maintain optimal osmotic pressure for sperm survival Buffering agents A. Source: Vesicular gland C. Function: guard against change in pH of semen, but not sufficient Energy substrates A. Metabolism: a. Fructose: used under anaerobic and aerobic conditions b. Sorbitol and GPC:used only aerobically c. GPC: utilized after splitted into choline and glycerylphosphate by an enzyme in female tract Other organic compounds A. Inositol and citric acid: considerably high, but not utilized.
Plasmalogen: a lipid reserved within sperm cell: used when other substrates are limiting A. Measurement of metabolism rate A. Under aerobic condition: 1.
O2 consumption 2. CO2 liberation 3 Methylene blue reduction time b. Under anaerobic condition: 1 pH reduction 2 Increase in lactic acid 3 Decrease in fructose B.
Relationship of fertile life to metabolic rate of sperm: a. Reduced metabolic rate : extend the storage life of semen; b. Reduced metabolic rate in epididymis: extend life of epididymal sperm c. Sperm in fresh ejaculate of semen: fertile for a few hours under high rate of metabolism Temperature A. High temperature: Increase metabolic rate and decrease life span of sperm B.
At body temperature: Sperm live for a few hours only: due to increase metabolism D. Higher metabolic rate: From pH of semen near neutrality 7. Deviations toward alkalinity or acidity: Can reduce metabolic rate, but buffering capacity of diluter is rather important because pH range to be altered without permanent impairment of sperm is narrow Osmotic pressure A. Semen diluted with isotonic diluter maintains maximum metabolic activity B.
Hypo- or hypertonic diluter: Can reduce metabolism but detrimental to sperm Concentration of spermatozoa A. Moderate dilution with buffered, isotonic medium containing fructose, before cooling: Not greatly alter metabolic rate, but extend sperm life C. Excessive dilution over 1,x : Depress motility and metabolic rate.
CO2 a. Epididymal sperm: Long life by high CO2 c. CO2 gassing in diluter: Effective for sperm preservation at room temperature B. Necessary for aerobic metabolism b. High level of O2: Toxic C. H2O2 production: Prevented by addition of catalase to diluter Antibacterial agents A. Penicillin and dihydrostreptomycin or neomycin: Used for control of bacterial growth, with no effects on sperm metabolism B.
Consider the previous concept that some men with low counts have highly fertile partners. Those couples make babies without our help. Only the couples where the man and the woman are both subfertile would come for donor sperm insemination. The idea of infertility treatment being a team sport is a useful concept to understanding that fertility disorders must always consider both parties.
Strict sperm morphology affects fertilization at IVF. The role of strict sperm morphology became evident in the s as illustrated in the following graphic. If his partner is also a 3 then they have infertility.
Azoospermia - no spermatozoa in the ejaculate. Cryptozoospermia - spermatozoa absent from fresh preparations but observed in a centrifuged pellet. Haemospermia haematospermia - presence of erythrocytes in the ejaculate. Leukospermia leukocytospermia, pyospermia - presence of leukocytes in the ejaculate above the threshold value.
Necrozoospermia - low percentage of live, and high percentage of immotile, spermatozoa in the ejaculate.
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Powerpoint presentation physiology labs semen analysis. If You're an Educator
Semen Analysis. Physiology of semen formation Indication of semen analysis Macroscopic examinationof semen Microscopic examination Antibody testing in semen Reference ranges. Physiology of Seminal fluid. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. Macroscopic Examination. Microscopical Examination. Sperm Count Decreased: vasectomy varicocele primary testicular failure Klinefelters secondary testicular failure congenital vas deferens obstruction endocrine causes prolactinemia, low testosterone.
Assessment of sperm morphology. Agglutination Reported when motile sperm stick to each other in a definite pattern. Head-head Tail-tail Head-tail Immunological cause of infertility. It is decreased in case of testosterone deficiency. So, fructose is used as fertility test.
Reference Ranges. Volume 2. Presentation Creator Create stunning presentation online in just 3 steps. Collect Leads Convert targeted audience into quality business leads. PRO Get powerful tools for managing your contents. Login Upload. Skip this Video. Loading SlideShow in 5 Seconds.. Semen Analysis PowerPoint Presentation. Download Presentation. Share Presentations. Email Presentation to Friend. Like Share Views. Uploaded on Jul 19, Related More by user.