Intake of oral supplements with the aim of a cutaneous antiaging effect are increasingly common. Hyaluronic acid HA is a promising candidate, as it is the key factor for preserving tissue hydration. In our practice study, we evaluated the effect of an oral HA preparation diluted in a cascade-fermented organic whole food concentrate supplemented with biotin, vitamin C, copper, and zinc Regulatpro Hyaluron on skin moisture content, elasticity, skin roughness, and wrinkle depths. Twenty female subjects with healthy skin in the age group of 45 to 60 years took the product once daily for 40 days. Different skin parameters were objectively assessed before the first intake, after 20 and after 40 days.
A new viscosupplement based on partially hydrophobic hyaluronic acid: a comparative study. Chondrocyte death and ECM loss may form a vicious cycle, with the progression of one aggravating the other, and there appears to be a correlation between the degree of cartilage damage and chondroptosis Effect of exogenous hyaluronic acid on joint function in experimentally induced equine osteoarthritis: Dosage titration studies. Hyaluronic acid viscosupplementation and osteoarthritis: current uses and future directions. The Clinical Report. Interstitial Cystitis. Food and Drug.
Motorcycle strap on. Uses and benefits
Ishihara, M. Furthermore, HA dietary supplements are expected to be effective anti-aging supplements because an Hyaluronan hyaluronic acid oral skin abstract ABC News program, which aired in Novemberstated that the key to longevity in a specific Japanese village was their HA-rich diet [ 8 ]. The Japanese Journal of Dermatology. In Hyzluronan field of cosmetics, the proper MW of HA was selected from a wide range of MW from hundreds of thousands to millionswhich was determined by the type of cosmetic product. Vet Med. This is in contrast to previous hyalutonic studies which showed that other HA forms were not absorbed and thus unavailable for use by the human body. Physiol Scand. Hyaluronan hyaluronic acid oral skin abstract of hyaluronic acid on the mechanical properties and water-holding capacity of Gel-type protein foods. The study was carried out ackd an open, uncontrolled monocentric study on the effects of this specific Hyalurojic preparation on 20 female healthy participants after 40 days of daily intake. Inside the probe, the penetration depth is determined by a noncontact Chandler escorts measuring system. Hyaluronan in skin: aspects of aging and its pharmacologic modulation. Because HA is highly viscous and retains moisture, it is responsible for facilitating smooth movement of the knee joint, and maintains the normal shape of the vitreous humor and moisturizes the skin.
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- The highest concentrations of hyaluronan occur in synovial fluid, vitreous body, skin and certain specialized tissues such as umbilical cord and rooster comb, during fetal development, and in tissue repair and regeneration.
- Due to its viscosity and moisturizing effect, HA is widely distributed as a medicine, cosmetic, food, and, recently marketed in Japan as a popular dietary supplement to promote skin moisture.
- Intake of oral supplements with the aim of a cutaneous antiaging effect are increasingly common.
Hyaluronic acid, also known as hyaluronan, is a clear, gooey substance that is naturally produced by your body. Hyaluronic acid has a variety of uses.
Roughly half of the hyaluronic acid in your body is present in your skin, where it binds to water to help retain moisture 1. However, the natural aging process and exposure to things like ultraviolet radiation from the sun, tobacco smoke and pollution can decrease its amounts in the skin 2 , 3.
Taking hyaluronic acid supplements may prevent this decline by giving your body extra amounts to incorporate into the skin 4 , 5. Doses of — mg per day for at least one month have been shown to significantly increase skin moisture and reduce dry skin in adults 3.
Hydrated skin also reduces the appearance of wrinkles, which may explain why several studies show that supplementing with it can make skin appear smoother 6 , 7. When applied to the surface of the skin, hyaluronic acid serums can reduce wrinkles, redness and dermatitis 8 , 9 , Some dermatologists even inject hyaluronic acid fillers to keep skin looking firm and youthful 11 , Hyaluronic acid helps wounds heal faster by regulating inflammation levels and signaling the body to build more blood vessels in the damaged area 13 , Applying it to skin wounds has been shown to reduce the size of wounds and decrease pain faster than a placebo or no treatment at all 15 , 16 , 17 , Hyaluronic acid also has antibacterial properties, so it can help reduce the risk of infection when applied directly to open wounds 19 , While the research on hyaluronic acid serums and gels is promising, there has been no research to determine whether hyaluronic acid supplements can provide the same benefits.
Hyaluronic acid is also found in the joints, where it keeps the space between your bones well lubricated When the joints are lubricated, the bones are less likely to grind against each other and cause uncomfortable pain. Hyaluronic acid supplements are very helpful for people suffering from osteoarthritis , a type of degenerative joint disease caused by wear and tear on the joints over time.
Taking 80— mg daily for at least two months has been shown to significantly reduce knee pain in people with osteoarthritis, especially those between the ages of 40 and 70 years old 23 , 24 , 25 , Hyaluronic acid can also be injected directly into the joints for pain relief.
However, an analysis of over 12, adults found only a modest reduction in pain and a greater risk of adverse effects Some research shows that pairing oral hyaluronic acid supplements with injections can help extend pain-relieving benefits and increase the amount of time between shots New research shows hyaluronic acid supplements may help reduce symptoms of acid reflux.
When acid reflux occurs, the contents of the stomach are regurgitated up into the throat, causing pain and damage to the lining of the esophagus.
Hyaluronic acid may help soothe the damaged lining of the esophagus and speed up the recovery process. One test-tube study found that applying a mixture of hyaluronic acid and chondroitin sulfate to acid-damaged throat tissue helped it heal much faster than when no treatment was used A second study showed that the same type of supplement was five times more effective at reducing acid reflux symptoms than a placebo Research in this area is still relatively new, and more studies are needed to replicate these results.
Nevertheless, these outcomes are promising. Approximately 1 out of 7 older adults suffer from symptoms of dry eye due to reduced tear production or tears evaporating too quickly Eye drops containing 0.
Contact lenses that contain slow-release hyaluronic acid are also being developed as a possible treatment for dry eye 36 , In addition, hyaluronic acid eye drops are frequently used during eye surgery to reduce inflammation and speed wound healing 38 , While applying them directly to the eyes has been shown to reduce dry eye symptoms and improve overall eye health, it is unclear whether oral supplements have the same effects.
To date, no studies have examined the effects of hyaluronic acid supplements on dry eye, but it may be a future area of research. New animal research has begun to investigate the effects of hyaluronic acid supplements on bone health. Two studies have found that hyaluronic acid supplements can help slow the rate of bone loss in rats with osteopenia, the beginning stage of bone loss that precedes osteoporosis 40 , Test-tube studies have also shown that high doses of hyaluronic acid can increase the activity of osteoblasts, the cells responsible for building new bone tissue 42 , While its effects on human bone health have not yet been studied, early animal and test-tube studies are promising.
This disorder causes abdominal pain and tenderness, along with a strong and frequent urge to urinate While the causes of interstitial cystitis are unknown, hyaluronic acid has been found to help relieve the pain and urinary frequency associated with this condition when inserted directly into the bladder through a catheter 46 , 47 , It's unclear why hyaluronic acid helps relieve these symptoms, but researchers hypothesize that it helps repair damage to bladder tissue, making it less sensitive to pain 49 , Studies have not yet determined whether oral hyaluronic acid supplements can increase amounts of it in the bladder enough to have the same effects.
One study in 60 people with osteoarthritis who took mg daily for one year reported no negative side effects However, its effects during pregnancy or breastfeeding have not been thoroughly studied, so these groups should be cautious and avoid supplementing with it.
There is also some evidence that cancer cells are sensitive to hyaluronic acid and taking supplements could make them grow faster 51 , For this reason, it is generally advised that people with cancer or a history of cancer avoid supplementing with it Hyaluronic acid injections into the skin or joints have a higher risk of side effects. However, negative reactions are mostly associated with the injection procedure, rather than hyaluronic acid itself 54 , Hyaluronic acid is well known for its skin benefits, especially alleviating dry skin, reducing the appearance of fine lines and wrinkles and speeding up wound healing.
Other notable applications include hyaluronic acid eye drops to relieve dry eye and inserting hyaluronic acid directly into the bladder via catheter to reduce pain. Overall, hyaluronic acid is a beneficial supplement for a variety of conditions, especially those related to skin and joint health.
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Arthritis is a condition that causes joint pain, stiffness and swelling. This article discusses 10 foods that can help relieve inflammation and fight…. Interstitial cystitis is a chronic inflammation of the bladder that can cause pelvic pain, frequent urination, and incontinence. Pain can be mild or…. Eating the right foods can help you age better, both on the inside and outside. Here are 11 foods that can help you look younger. Promotes Healthier, More Supple Skin. Can Speed Wound Healing.
Soothe Acid Reflux Symptoms. Relieve Dry Eye and Discomfort. Preserve Bone Strength. Could Prevent Bladder Pain. Possible Side Effects and Precautions. Do Glucosamine Supplements Work for Arthritis? Read this next. The 12 Best Foods for Healthy Skin. Interstitial Cystitis.
External link. Arch Dermatol Res. This optical measuring system consists of a light source and a light receptor, as well as 2 prisms facing each other, which project the light from transmitter to receptor. Forgot password? Measurements of corneometry, elasticity, skin roughness, and wrinkle depths at baseline, after 20 and after 40 days were compared using repeated-measures analysis of variance ANOVA. Features of skin aging, such as wrinkle formation, occur primarily because of irradiating ultraviolet rays from sunlight; thus, HA can also be expected to have anti-aging effects, such as improving skin texture and reducing wrinkles. Both the partially depolymerized and the fully intact HA were distributed to the skin.
Hyaluronan hyaluronic acid oral skin abstract. Introduction
Oral hyaluronan relieves wrinkles: a double-blinded, placebo-controlled study over a week period
Hyaluronan HA has critical moisturizing property and high water retention capacity especially for human skin. This study aimed to evaluate the effect of oral intake of HA. The mean molecular weight MW of HA is 2 k and k. The skin wrinkles were evaluated by image analysis of skin wrinkle replicas, and their skin condition was evaluated using a questionnaire survey.
During the study period, the HA groups showed better level of the whole sulcus volume ratio, wrinkle area ratio, and wrinkle volume ratio than the placebo group.
After 8 weeks of ingestion, the HA k group showed significantly diminished wrinkles compared with the placebo group. Skin luster and suppleness significantly improved after 12 weeks in all groups compared with the baseline. Hyaluronan HA is a body component that is present in every connective tissue and organ, such as skin, synovial fluid, blood vessels, serum, the brain, cartilage, heart valves, and the umbilical cord.
Wrinkles of the skin are formed under the influence of various factors, such as aging, ultraviolet UV light, and dryness. In particular, the degradation of collagen and HA by UV damage causes wrinkles. HA content in the skin is considered to be related to the factors that cause wrinkles. The effect on skin wrinkles through the use of oral HA is expected because the decrease of skin damage leads to relieving of skin wrinkles.
In addition, dry skin was improved by oral ingestion of HA. For example, a year-old person has only one quarter of the amount of HA in their skin compared with a year-old person. With respect to skin wrinkles, there are some surgical techniques that use fillers as a symptomatic treatment. However, the cost of treatment is high, and there are risks of pain and swelling.
Accordingly, the demand for dietary supplements grows because supplements are easy to take continuously and support the inner body. Several studies reported some of the effects that dietary supplements have on keeping skin healthy. Two clinical trials of oral HA have been performed.
It is not confirmed whether the anti-wrinkle effect of oral HA is different depending on its MW or amount of intake. However, it is known that the bioavailability of HA differs depending on the molecular weight. HA 2 k is slightly more soluble than HA k. Informed consent was obtained in writing from each subject prior to enrollment in the study. Sixty volunteers were selected.
Our exclusion criteria are shown in Table 1. The selected subjects were assigned randomly to three groups, i. After grouping, a double-blind, placebo-controlled study was conducted. The placebo group received two capsules mg microcrystalline per capsule , and the HA 2 k group received two capsules 60 mg HA with microcrystalline cellulose, total mg per capsule each day. The HA k group received two capsules 60 mg HA with microcrystalline cellulose, total mg per capsule each day.
There were no differences in the appearance and taste of the HA and placebo capsules. The capsules were manufactured by Aliment Industry Co. Ltd Yamanashi, Japan. This study was conducted by Kewpie Corporation, who carried out a randomized, double-blind, placebo-controlled trial. Subjects were randomly assigned to either the HA 2 k, HA k, or placebo group before starting the study according to stratified randomization based on age by the study coordinator, MO.
Allocation was documented in an electronic file accessible only to the study coordinator. The study investigators, NO and HK, who did not know the allocation, handed the samples which are no difference in appearance to the subjects.
The subjects consumed two capsules each day for 12 consecutive weeks. The skin evaluation was conducted prior to ingestion and after 4, 8, and 12 weeks of treatment. The evaluation of skin conditions was conducted using the image analysis of skin wrinkle replicas and a questionnaire survey.
This clinical trial was conducted in accordance with the principles of the Declaration of Helsinki and was approved by the institutional review board Shinkohkai Medical Corporation Institutional Review Board, Tokyo, Japan. Collection and analysis of the replica were carried out as follows, according to the Guideline for Evaluation of Anti-wrinkle Products Japanese Cosmetic Science Society. On evaluation day, the subjects applied the skin replica agent Asahi Techno Lab Corporation, Kanagawa, Japan on the corner of their eye.
After it had sufficiently dried, the participants peeled the replicas off the skin and allowed the replica to dry again. The amount of change relative to the initial value of the mean value in each item was compared among the groups with respect to the placebo group. A questionnaire survey was completed by the subjects to assess the luster, suppleness, and wrinkles of the facial skin using the five-point evaluation scale Table 2 at the following times: prior to ingestion and after 4, 8, and 12 weeks of ingestion.
For the skin luster and suppleness, one is the worst and five is the best. For the wrinkles, one is least wrinkles; not worry, and five is most wrinkles; most care.
The amount of change relative to the initial value of the score mean value in each item was compared among the groups with respect to the placebo group. A questionnaire survey on adverse effects, such as allergic reactions, diarrhea, and abdominal pain, was conducted during the study.
For the questionnaire survey, a Wilcoxon signed-rank sum test was used to compare the initial value and each measurement point. For the evaluation of the wrinkle replica, two people from the placebo group and four people from the HA 2 k group and the HA k group which originally had 60 people did not complete the trial due to personal reasons. A total of 50 people were assessed as shown in Table 3. Temporal changes in the whole sulcus volume ratio, wrinkle area ratio, and wrinkle volume ratio during the study period are shown in Table 4.
In the placebo group, the whole sulcus volume ratio, wrinkle area ratio, and wrinkle volume ratio had a higher value but were not significant compared with that prior to ingestion.
In the HA 2 k group and HA k group, the whole sulcus volume ratio, wrinkle area ratio, and wrinkle volume ratio showed a consistently low value compared with that prior to ingestion. Changes in the analysis results of the wrinkle replice with time after ingestion of hyaluronan or the placebo.
The amount of change in the whole volume ratio, wrinkle area ratio, and wrinkle volume ratio during the test period is shown in Figure 1. The value before ingestion did not affect the results, therefore a covariance analysis analysis of covariance [ANCOVA] was not performed. Changes from baseline in the analysis results of the wrinkle replica with time after ingestion of HA or the placebo. Subjective symptoms about the luster, suppleness, and wrinkles were evaluated using a questionnaire.
In the questionnaire evaluation, one person from the placebo group and two from both the HA 2 k group and the HA k group of the original 60 people did not complete the questionnaire because of personal reasons.
A total of 55 people participated. For the luster, the HA 2 k group and the HA k group showed an increase of scores compared with the placebo group at all measured points after 4, 8, and 12 weeks. For wrinkles, the placebo group, HA 2 k group, and HA k group showed a significantly decreased score after 8 and 12 weeks compared to prior to ingestion Figure 2.
Changes in the subjective skin condition with time after ingestion of HA or the placebo. The Wilcoxon signed-rank test was used to compare the three groups.
In this study, a questionnaire survey and observation were conducted, and there were no adverse events. We could not confirm the change of the skin surface after ingestion for the subjects who ingested placebo; however, the skin surface of the subjects who ingested HA tended to improve.
As an example of each group, microscopic images of the outer corner of the eye are shown prior to ingestion and after 8 weeks of ingestion Figure 3. Notes: Microscopic images of the outer corner of the eye prior to ingestion and after 8 weeks of ingestion are shown for each group. They were given the mg HA of MW 2 k or k per day for 12 weeks.
As a result, in the replica image analysis evaluation of wrinkles and the questionnaire survey of subjective symptoms in the skin, it was confirmed that ingestion of HA improved the luster and suppleness of the skin and caused a decrease in skin wrinkles. In the placebo group, the whole sulcus volume ratio, wrinkle area ratio, and wrinkle volume ratio increased at 8 weeks probably because this study was conducted in dry season from January to April.
In the replica image analysis of both the HA 2 k group and HA k group, the transition of the amount of change in the whole volume ratio, wrinkle area ratio, and wrinkle volume ratio maintained low values compared with the placebo group and shown suppressed wrinkles compared with the time prior to ingestion. The amount of change in the wrinkle volume ratio significantly decreased at 8 weeks after ingestion in the HA k group compared to the placebo group Figure 1.
A previous randomized controlled trial RCT study showed ingestion of the HA of MW 38 k suppressed wrinkles significantly compared with the placebo group. Considering the role of HA in the skin, it is suggested that migration of HA to the skin acts on the wrinkled skin as follows:.
There are fibroblasts in the dermis of the skin. In these fibroblasts, collagen fibers, elastin fibers, and HA are synthesized. In vitro, HA prompts the proliferation of fibroblasts of the dermis, and it was confirmed that intake of HA promoted HA synthesis in fibroblasts.
In addition, because the fibroblast cells are growing, it is possible to suppress wrinkles by promoting collagen synthesis. The epidermis above the dermis consists of the stratum corneum, the granular layer, spinous layer, and the basal layer beginning from the upper layer. The necessary HA in the epidermis is synthesized in the keratinocytes of the basal layer. In addition to this, it is presumed that the suppression of wrinkle formation is involved in several factors HA causes.
From these mechanisms, it is believed to have led to an efficacy against wrinkles, the improvement of subjective symptoms in the skin, and the improvement of skin conditions through visual observation in this study.
Because the turnover for the skin is said to be 28 days, functionality through oral ingestion of the HA will require long-term continuous intake. The previous study of the MW 38 k and 75 k HA showed the effect on wrinkles after ingestion of 4 and 8 weeks compared with placebo group. There is no study about the effect of HA under 38 k or over 75 k on wrinkles. This study clarified that the MW 2 k and k HA had effects on wrinkles. Therefore, we confirmed that HA between the MW 2 k and k had effects on wrinkles.
It was not studied for the minimum effective dose. It was also not verified whether there is similar effectiveness in MWs other than 2 k and k. In addition, it is not certain that changes of HA content in the skin were caused through oral ingestion of HA and what affect HA has regarding the wrinkle-inducing factor. Regarding these issues, further research is required in the future. From the above, HA consumption is expected to be used as a method to maintain healthy skin.
We gratefully acknowledge the individuals who participated in the study. This study was funded by the Kewpie Corporation. Mariko Oe, Mr. Seigo Sakai, Mr.